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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4

Fig. 2

HemoHIM induced the phenotypic maturation of BMDCs. a BMDCs were treated with the indicated concentrations of LPS or HemoHIM for 24 h. Flow cytometry was used to assess the expressions of co-stimulatory molecules by CD11c+-gated BMDCs. The mean fluorescence intensities (M.F.I) and the percentages of positive cells are shown in each panel. b IL-1β, IL-6, IL-12p70, and TNF-α levels in HemoHIM-treated BMDCs were analyzed by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. untreated BMDCs. c Antigen uptake activities at 37 °C were assessed based on dextran-FITC uptake using flow cytometry. The percentages of dextran-FITC+ CD11c+ cells are indicated. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. untreated BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment

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