Fig. 2

Effects of Polyphyllin VII (PP7) on mitochondrial membrane potential and intracellular reactive oxygen species (ROS) in HepG2 Cells. a HepG2 cells were treated with indicated concentrations of PP7 for 24 h and photographed using the InCell 2000 confocal microscope after JC-1 staining. b Quantitative analysis of green (FITC)/red (CY3) fluorescence in HepG2 cells, ** p < 0.01, compared to control (CTL). Observation of intracellular ROS generation by DCFH-DA staining in HepG2 cells after treated with indicated doses of PP7 for 24 h (c, e) or treated with 1.32 μM PP7 for different times (d, f). ** p < 0.01, compared to CTL. Data are both represented as means ± SD from 3 independent experiments