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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Fructus mume extracts alleviate cognitive impairments in 5XFAD transgenic mice

Fig. 4

F. mume treatment increased hippocampal ChAT expression in 5XFAD mice. a Hippocampal or cortical ChAT expression level was analyzed by western blot (normalized to non-transgenic + vehicle). b The expression levels of hippocampal ChAT were significantly reduced in vehicle-treated 5XFAD mice compared with vehicle-treated non-transgenic mice (*p < 0.05). F. mume treatment mitigated this reduction (# p < 0.05) (n = 6 per group). c No significant differences in expression levels of cortical ChAT were found between the groups (n = 8 – 9 per group). d Representative ChAT-positive neurons in the medial septum (MS) and substantia innominata (SI) are shown. Scale bar = 100 μm. e The number of ChAT-positive neurons in the MS and vertical limb of the diagonal band (VDB), which provide cholinergic innervation to the hippocampus, was counted (n = 6 per group). f The number of ChAT-positive neurons in the SI and horizontal limb of the diagonal band (HDB), which provide cholinergic innervation to the cortex, was counted (n = 4 per group). No significant differences were observed in the number of ChAT-positive neurons in the basal forebrain between the groups. All the data are presented as the mean ± SEM

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