Fig. 3

Effect of EPK on adipogenesis in 3 T3-L1 preadipocytes. 3 T3-L1 preadipocytes were incubated in medium containing insulin (1.0 μg/mL) with or without the indicated concentrations of EPK or CC. a Total RNA was extracted from 3 T3-L1cells treated with EPK and used for RT-PCR analysis of PPARγ, C/EBPα, GPDH, and β-actin. b Total proteins prepared from EPK-treated 3 T3-L1 cells were subjected to western blot analysis for AMPK, p-AMPK, PPARγ, C/EBPα, adiponectin, FABP, SREBP-1, HMGCR, and β-actin. c 3 T3-L1 cells were transfected with AMPK-siRNA for 48 h and treated with or without EPK (50 μg/mL) for 24 h and subjected to western blot analysis for AMPK, p-AMPK, PPARγ, SREBP-1, and β-actin. d Total proteins prepared from EPK (50 μg/mL) or CC (2 μM)-treated 3 T3-L1 cells for 6 h were subjected to western blot analysis for AMPK, p-AMPK, PPARγ, FAS, SREBP-1, HMGCR, and β-actin. e Cells were fixed and stained with Oil-Red-O. The Oil-Red-O-stained adipocytes were photographed at 100× magnification under a microscope. Preadipocyte (P), compound C (CC)