Fig. 2
Effect of aqueous clay leachates on M. ulcerans growth in vitro. M. ulcerans was exposed to 1 ml of supplemented M7H9 broth (control), sterile deionized H2O, or clay leachates. At specified times, samples were plated on supplemented M7H10 agar and incubated at 32 °C to determine viable CFU. Data represent three independent experiments performed in triplicate, and results are expressed as mean ± SD. Statistically significant differences, compared to dH2O-incubated samples on corresponding days, are shown. ***, p < 0.001; ****, p < 0.0001; ANOVA, Tukey’s multiple comparisons