Table 2 Forward and reverse primers and expected sizes of AR, 5α-R enzymes and β-actin
Name | Primer pair
| Expected size (bp) |
|---|---|---|
Androgen receptor (AR) | F: 5' CGTGCGCGAAGTGATCCAGAA 3' | 811 |
GenBank:NM_000044.3 | R: 5' TGCGCTGTCGTCTAGCAGAGAA 3' | |
5α-Reductase type 1 (5α-R1) | F: 5' ACTGCATCCTCCTGGCCATGTTC 3' | 380 |
GenBank:NM_001047.2 | R: 5' GGCATAGCCACACCACTCCATGA 3' | |
5α-Reductase type 2 (5α-R2) | F: 5' AAGCACACGGAGAGCCTGAA 3' | 450 |
GenBank:NM_000348.3 | R: 5' GCCACCTTGTGGAATCCTGTAGC 3' | |
β-actin (internal control) | F: 5' ATGATGATATCGCCGCGCTC 3' | 584 |
GenBank:NM_001101.3 | R: 5' GCGCTCGGTGAGGATCTTCA 3' |
