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Fig. 7 | BMC Complementary and Alternative Medicine

Fig. 7

From: Flavonoids of Polygonum hydropiper L. attenuates lipopolysaccharide-induced inflammatory injury via suppressing phosphorylation in MAPKs pathways

Fig. 7

Effects of FNP on iNOS and COX-2 protein expression in LPS-stimulated RAW264.7 cells. Notes: The cells were pretreated with different concentrations (40 μg/mL, 80 μg/mL) of FNP for 2 h or 4 h and stimulated with 1 μg/mL of LPS for 24 h. Total cellular proteins (50 μg) were separated by SDS-PAGE, then transferred to PVDF membrane and detected by Western blot analysis. Quantification of iNOS and COX-2 protein expression was normalized to β-actin using a densitometer. Each column represented as the means ± SD from three independent experiments. ## P <0.01 vs control group; * P <0.05 vs LPS group; ** P <0.01 vs LPS group

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