Fig. 5From: Flavonoids of Polygonum hydropiper L. attenuates lipopolysaccharide-induced inflammatory injury via suppressing phosphorylation in MAPKs pathwaysCytotoxic effects of FNP in RAW264.7 cells (a) and the effects of FNP on LPS-induced NO (b), and ROS (c) productions in RAW264.7 macrophages. Notes: The cells were incubated for 24 h with 1 μg/mL of LPS in the absence or presence of FNP (20, 40, and 80 μg/mL). FNP was added 1 h before incubation with LPS. Cell viability assay was performed by using MTT assay. Nitrite concentration in the medium was determined by using Griess reagent. ROS production was determined by fluorescent probe DCFH-DA. Each column represented as the means ± SD from three independent experiments. ## P <0.01 vs control group; * P <0.05 vs LPS group; ** P <0.01 vs LPS groupBack to article page