Table 2 Gradient elution program for HPLC
Time (min) | Flow (mL/min) | % acetonitrile (A) | % ammonium acetate buffer, PH 4.5 (B) |
|---|---|---|---|
0~5 | 1 | 5 | 95 |
5~25 | 1 | 18 | 82 |
25~30 | 1 | 18 | 82 |
30~45 | 1 | 32 | 68 |
45~60 | 1 | 70 | 30 |
Time (min) | Flow (mL/min) | % acetonitrile (A) | % ammonium acetate buffer, PH 4.5 (B) |
|---|---|---|---|
0~5 | 1 | 5 | 95 |
5~25 | 1 | 18 | 82 |
25~30 | 1 | 18 | 82 |
30~45 | 1 | 32 | 68 |
45~60 | 1 | 70 | 30 |