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Table 1 Human skin primary irritation test

From: Inhibition of melanogenesis by Gaillardia aristata flower extract

No. Test Material 48 h 72 h Reaction gradeb
± 1+ 2+ 3+ 4+ ± 1+ 2+ 3+ 4+ 48 h 72 h Mean
1 Squalene -a - - - - - - - - - 0 0  
2 GAE (0.1 %) - - - - - - - - - - 0 0  
  1. aNo reaction; bReaction grade = Σ [{Grade × No. of Responders}/{4(Maximum grade) × 30 (Total subject)}] × 100 × (1/2)