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Table 1 Human skin primary irritation test

From: Inhibition of melanogenesis by Gaillardia aristata flower extract

No.

Test Material

48 h

72 h

Reaction gradeb

±

1+

2+

3+

4+

±

1+

2+

3+

4+

48 h

72 h

Mean

1

Squalene

-a

-

-

-

-

-

-

-

-

-

0

0

 

2

GAE (0.1 %)

-

-

-

-

-

-

-

-

-

-

0

0

 
  1. aNo reaction; bReaction grade = Σ [{Grade × No. of Responders}/{4(Maximum grade) × 30 (Total subject)}] × 100 × (1/2)