Fig. 3
From: Inhibition of melanogenesis by Gaillardia aristata flower extract
Evaluations the effects of GAE on cellular tyrosinase activity by tyrosinase inhibition (a), zymography (b) and tyrosinase luciferase reporter assay (c) in Human melanocyte. Human melanocytes were treated with the indicated concentrations of GAE for 5 days and then the cellular tyrosinase activity was measured. The results are expressed relative to controls. Data are mean ± standard deviation. *p < 0.05 compared with the vehicle-treated group, **p < 0.01 compared with the vehicle-treated group (n = 3). Cell viability (d) and melanin content (e) were determined in B16F10 cells treated with GAE. Before measuring the melanin content, cells were observed using a contract microscope and photographed using an attached digital camera. Cellular tyrosinase activity was measured using tyrosinase inhibition (g), L-DOPA zymography analysis (h) and tyrosinase luciferase reporter assay (i). The results are expressed relative to controls. Data are mean ± standard deviation. ##p < 0.01 compared with the vehicle-treated group, *p < 0.05 compared with the α-MSH-treated group, **p < 0.01compared with the α-MSH-treated group (n = 3)