Fig. 3
Effect of butein on serum-stimulated B16F10 cell motility. a B16F10 cells (5 × 104) were seeded onto 6-well plates for 24 hr and starved for another 24 hr with serum-free DMEM. Cells were subjected to injury by scratching with a plastic pipette tip (200 μl). The cells were then treated for 24 hours with or without serum or butein. The quantitative of migratory cell was shown in b. Data are presented as percentages of control (mean ± S.E.M., n = 3). **, P < 0.01 and ***,P < 0.001 versus control group