Fig. 2
Effects of PGL and PGPC on PMA + Iono-stimulated ERK1/ERK2 phosphorylation in splenic T-cells. Data were quantified by densitometry and expressed as phosphorylated /non phosphorylated ERK1/ERK2 ratio. Splenic T-cells (5 × 106 cells/ml), before determination of MAP kinase phosphorylation, were incubated for 6 h in RPMI 1640 medium without serum, and treated with increasing concentrations (0 to 40 μg/ml) of PGL in (a) and PGPC in (b). After 5 min of incubation, cells were stimulated with PMA (50 nM) and Iono (500 nM) for another 30 min at 37 °C. Cells were lysed and phosphorylated MAP kinases were detected performed as described in Materials and Methods. Results are expressed as arbitrary units in bar graphs. *Represents P < 0.001 as compared to PMA + Iono-stimulated T-cells in the absence of PGL (a) and PGPC (b). NS = insignificant differences. p values were obtained by one-way ANOVA, followed by Fisher’s LSD test