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Table 1 Results and analysis of the orthogonal test

From: Isolation of a novel bio-peptide from walnut residual protein inducing apoptosis and autophagy on cancer cells

No.

A

B

C

D

IR (%)a

Temperature (°C)

Time (h)

S (%)

E/S (%)

1

55

3

3

8

66.13 ± 0.78

2

55

4

4

10

69.57 ± 1.13

3

55

5

5

12

63.73 ± 1.83

4

60

3

4

12

66.41 ± 0.85

5

60

4

5

8

70.55 ± 0.69

6

60

5

3

10

68.21 ± 0.50

7

65

3

5

10

68.34 ± 2.15

8

65

4

3

8

60.53 ± 1.90

9

65

5

4

12

64.81 ± 1.79

K1

66.48 ± 2.94

66.96 ± 1.20

64.96 ± 3.97

65.74 ± 5.02

 

K2

68.39 ± 2.08

66.89 ± 5.52

66.93 ± 2.42

68.71 ± 0.75

 

K3

64.56 ± 3.91

66.58 ± 2.34

67.54 ± 3.48

65.98 ± 1.35

 

Best level

60 °C

3 h

5 %

10 %

 

R

3.67

0.60

1.82

3.88

 

R order

D > A > C > B

  1. aMCF-7 inhibitory rate