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Table 1 Results and analysis of the orthogonal test

From: Isolation of a novel bio-peptide from walnut residual protein inducing apoptosis and autophagy on cancer cells

No. A B C D IR (%)a
Temperature (°C) Time (h) S (%) E/S (%)
1 55 3 3 8 66.13 ± 0.78
2 55 4 4 10 69.57 ± 1.13
3 55 5 5 12 63.73 ± 1.83
4 60 3 4 12 66.41 ± 0.85
5 60 4 5 8 70.55 ± 0.69
6 60 5 3 10 68.21 ± 0.50
7 65 3 5 10 68.34 ± 2.15
8 65 4 3 8 60.53 ± 1.90
9 65 5 4 12 64.81 ± 1.79
K1 66.48 ± 2.94 66.96 ± 1.20 64.96 ± 3.97 65.74 ± 5.02  
K2 68.39 ± 2.08 66.89 ± 5.52 66.93 ± 2.42 68.71 ± 0.75  
K3 64.56 ± 3.91 66.58 ± 2.34 67.54 ± 3.48 65.98 ± 1.35  
Best level 60 °C 3 h 5 % 10 %  
R 3.67 0.60 1.82 3.88  
R order D > A > C > B
  1. aMCF-7 inhibitory rate