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Table 1 MTT reduction cytotoxic assay for evaluation of H. abyssinica and its isolated compounds for their inhibition % against 3T3 cell line

From: Immunosuppressive phenolic compounds from Hydnora abyssinica A. Braun

Conc. in μg/mL

Compound (1)

Compound (2)

Compound (3)

Ethanolic extract

50

17.8 + 3.3

17.2 + 3.7

15.8 + 5.1

12.4 + 4.2

25

14.4 + 4.1

14.2 + 2.8

12.5 + 4.3

11.5 + 3.5

12.5

10.8 + 3.6

12.1 + 3.1

11.4 + 4.1

10.2 + 2.7

6.25

8.1 + 2.8

4.0 + 1.3

11.9 + 3.8

8.7 + 2.3

3.13

1.4 + 0.3

3.4 + 0.4

4.7 + 1.1

-

Triton X

66.7 + 11.6

  1. This table indicates the activity of HA ethanolic extract and isolated compounds on the viability of 3T3 cell line after 72 h incubation with and without five concentrations (6.25–100 μg/mL) of the plant extract and (3.13–50 μg/mL) for isolated compounds. Triton X 0.5 % was used as control positive. Cells viability was evaluated by MTT reduction to the blue colored formazan in living cells which was detected by measuring absorbance at 540 nm against blank samples. The % inhibition was calculated as: % Inhibition = [(AControl − ASample)/AControl] × 100
  2. Where AControl is the absorbance of the negative control and ASample the absorbance of tested samples or standard. Readings are presented in mean ± SD of three repeats