Fig. 8From: Tuscan black kale sprout extract bioactivated with myrosinase: a novel natural product for neuroprotection by inflammatory and oxidative response during cerebral ischemia/reperfusion injury in ratEffects of Bioactive TBK-SE on Nrf2 expression. Negative staining for Nrf2 was observed in cortex (a), hippocampus (b), brainstem (c) and cerebellum (d) of CIR rats. On the contrary, positive staining for Nrf2 was observed in cortex (e), hippocampus (f), brainstem (g) and cerebellum (h) from rats treated with bioactive TBK-SE. Also, western blot analysis showed a basal level of Nrf2 expression in cerebellum samples obtained from CIR rats. Bioactive TBK-SE treatment significantly increased Nrf2 expression (i). GAPDH was used as internal control. *p < 0.05 vs CIRBack to article page