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Table 5 The total protein, lipid peroxidation and antioxidant enzyme activity of chicken hepatocytes pretreated with phenolic-enriched fraction (PEF) and exposed to AFB1

From: Cytoprotective effect of palm kernel cake phenolics against aflatoxin B1-induced cell damage and its underlying mechanism of action

Items AFB1 (5 μM) S.E.M
PEF (μg/ml) Control+
0 5 10 20 40
Total protein (mg ml−1) 0.6b 0.6b 0.6b 0.8a 0.8a 0.8a 0.02
Lipid peroxidation (nM MDA mg−1 protein) 7.5a 6.9ab 5.6c 4.3d 3. 8de 4.6d 0.46
SOD activity (U mg−1 protein) 4.2d 4.8cd 5.7bc 6.3b 7.8a 6.7ab 0.8
CAT activity (U mg−1 protein) 3.8de 4.2d 4.9bcd 5.7b 6.9a 5.5bc 0.47
GR activity (U mg−1 protein) 0.2c 0.2c 0.4b 0.5a 0.5a 0.5a 0.06
  1. All cells were pre-treated with different concentrations of PEF for 24 h, then the media were replaced with a medium containing 5 μM of AFB1 and the cells were incubated for another 48 h
  2. MDA Malondialdehyde as lipid peroxidation biomarker
  3. Control+: 10 μM or 1.7 μg/ml gallic acid
  4. SOD Superoxide dismutase
  5. CAT Catalase
  6. GR Glutathione reductase
  7. S.E.M Standard error of the means
  8. Means (n = 3) with different superscripts (a,b,c,d,e) within a row are significantly different (p < 0.05)