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Table 5 The total protein, lipid peroxidation and antioxidant enzyme activity of chicken hepatocytes pretreated with phenolic-enriched fraction (PEF) and exposed to AFB1

From: Cytoprotective effect of palm kernel cake phenolics against aflatoxin B1-induced cell damage and its underlying mechanism of action

Items

AFB1 (5 μM)

S.E.M

PEF (μg/ml)

Control+

0

5

10

20

40

Total protein (mg ml−1)

0.6b

0.6b

0.6b

0.8a

0.8a

0.8a

0.02

Lipid peroxidation (nM MDA mg−1 protein)

7.5a

6.9ab

5.6c

4.3d

3. 8de

4.6d

0.46

SOD activity (U mg−1 protein)

4.2d

4.8cd

5.7bc

6.3b

7.8a

6.7ab

0.8

CAT activity (U mg−1 protein)

3.8de

4.2d

4.9bcd

5.7b

6.9a

5.5bc

0.47

GR activity (U mg−1 protein)

0.2c

0.2c

0.4b

0.5a

0.5a

0.5a

0.06

  1. All cells were pre-treated with different concentrations of PEF for 24 h, then the media were replaced with a medium containing 5 μM of AFB1 and the cells were incubated for another 48 h
  2. MDA Malondialdehyde as lipid peroxidation biomarker
  3. Control+: 10 μM or 1.7 μg/ml gallic acid
  4. SOD Superoxide dismutase
  5. CAT Catalase
  6. GR Glutathione reductase
  7. S.E.M Standard error of the means
  8. Means (n = 3) with different superscripts (a,b,c,d,e) within a row are significantly different (p < 0.05)