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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Chungsimyeonja-eum inhibits inflammatory responses in RAW 264.7 macrophages and HaCaT keratinocytes

Fig. 5

Changes in TNF-α and IFN-γ-stimulated STAT1 phosphorylation in CSYJE-treated HaCaT cells. a The expression of STAT1 phosphorylation was measured using cells cotreated with CSYJE, and TNF-α and IFN-γ (each 10 ng/mL, TI) for 24 h. Silymarin (12.5, or 25 μg/mL) was used as a positive control. b Cellular localization of STAT1 was examined by immunofluorescence staining. Cells were co-treated with CSYJE (500 μg/mL), and TI for 30 min. The cells were fixed in 4 % (v/v) methanol-free formaldehyde solution (pH 7.4), stained with anti-STAT1 (red). The stained cells were mounted in medium containing DAPI (blue) and visualized under an Olympus FLUOVIEW FV 10i confocal microscope

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