Fig. 4

Histopathological findings showing the preventive effect of dietary RPE on DNCB-induced AD-like skin lesions in mice. a Skin sections (4 μm thick) were stained with hematoxylin & eosin (upper panel), toluidine blue (middle panel) and Giemsa (lower panel). ED, epidermis; Dr, dermis; magnification, 200 ×; scale bars, 50 μm. b Epidermal thickness was analyzed in the hematoxylin & eosin-stained sections. c The number of mast cells was analyzed in the toluidine blue-stained sections. d The number of eosinophils was analyzed in the Giemsa-stained sections. **P < 0.01 compared with the normal group, and ^†P < 0.05, ^††P < 0.01 compared with the DNCB control group (n = 6)