Fig. 1
Experimental protocol for rice prolamin extract (RPE) feeding and induction of atopic dermatitis. BALB/c mice were fed diet supplemented with 0, 0.05 % and 0.1 % RPE for 6 weeks. For the last 2 weeks, the back skin of mice was applied repeatedly with DNCB to induce AD. For DNCB sensitization, a 1 cm2 gauze-attached patch was applied with 0.1 ml of 1 % DNCB and attached to the back skin for 2 days on day 0 and 3. And for the following challenge process, the gauze-attached patch was applied with 0.1 ml of 0.2 % DNCB and attached to the sensitized area for a day on day 7 and 10. The mice were sacrificed on day 14 to evaluate the effects of DNCB and RPE treatments