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Table 2 Mean of Growth inhibition zone diameter (mm) of ethanol extract of Golnar and its fractions (n = 3)

From: Total phenolic and flavonoid content and antibacterial activity of Punica granatum L. var. pleniflora flowers (Golnar) against bacterial strains causing foodborne diseases

Extract concentration

500 mg/ml

250 mg/ml

125 mg/ml

62.5 mg/ml

31.25 mg/ml

15.62 mg/ml

7.8 mg/ml

3.9 mg/ml

1.95 mg/ml

Microorganism

Staphylococcus aureus ATCC 25923

M

32 ± 0

30 ± 0.6

29 ± 1

28 ± 1

27 ± 0

26 ± 0.6

25 ± 0.6

24 ± 1

23 ± 0

W

34 ± 0.6

32 ± 1

31 ± 1

30 ± 0.6

28 ± 1

27 ± 0.6

26 ± 1

25 ± 0.6

24 ± 0.6

EA

31 ± 1

28 ± 1

27 ± 1

26 ± 0.6

24 ± 1

22 ± 0

18 ± 0

15 ± 1

13 ± 0

C

19 ± 1

16 ± 0

14 ± 0.6

13 ± 0.6

-

-

-

-

-

EE

32 ± 0

30 ± 0

28 ± 0.6

27 ± 1

25 ± 1

24 ± 1

23 ± 1

22 ± 0

21 ± 0.6

Bacillus cereus PTCC 1247

M

29 ± 0

21 ± 0

19 ± 1

17 ± 1

16 ± 1

15 ± 0.6

14 ± 0.6

13 ± 0.6

12 ± 0.6

W

28 ± 1

23 ± 1

22 ± 0

19 ± 0

18 ± 0

16 ± 0

14 ± 0.6

13 ± 0.6

12 ± 0.6

EA

29 ± 0.6

23 ± 0.6

20 ± 0.6

14 ± 1

13 ± 1

12 ± 0

11 ± 0

10 ± 0

-

C

17 ± 0

13 ± 1

12 ± 1

11 ± 1

10 ± 0.6

-

-

-

-

EE

28 ± 0.6

25 ± 0.6

23 ± 0

19 ± 0

18 ± 1

16 ± 1

15 ± 1

12 ± 0

11 ± 0

Listeria monocytogenes ATCC 7644

M

30 ± 0

26 ± 1

22 ± 0.6

19 ± 0.6

17 ± 0.6

15 ± 0

14 ± 0

11 ± 0

10 ± 0.6

W

30 ± 0.6

25 ± 1

23 ± 1

20 ± 0

17 ± 0

15 ± 0

14 ± 0.6

12 ± 0.6

11 ± 0.6

EA

28 ± 0

22 ± 0

20 ± 1

17 ± 0.6

13 ± 1

-

-

-

-

C

18 ± 1

14 ± 1

11 ± 0.6

-

-

-

-

-

-

EE

32 ± 1

28 ± 1

26 ± 1

26 ± 0

22 ± 0

18 ± 0

14 ± 0.6

13 ± 0.6

11 ± 0.6

Shigella dysantriae PTCC 1188

M

32 ± 0.6

29 ± 0.6

28 ± 0.6

28 ± 0

27 ± 0

26 ± 1

25 ± 1

24 ± 0

23 ± 0.6

W

30 ± 1

28 ± 0.6

27 ± 0.6

26 ± 0

25 ± 0

24 ± 0

22 ± 0.6

20 ± 1

19 ± 1

EA

30 ± 0

27 ± 0

24 ± 0.6

21 ± 0.6

20 ± 0.6

19 ± 0

17 ± 0

15 ± 0.6

12 ± 0.6

C

18 ± 0.6

15 ± 1

13 ± 1

12 ± 1

-

-

-

-

-

EE

30 ± 1

27 ± 0

25 ± 0

24 ± 0.6

23 ± 1

21 ± 1

20 ± 1

19 ± 0

18 ± 0

Salmonella typhi ATCC 19430

M

31 ± 0.6

24 ± 0

21 ± 0

18 ± 0.6

14 ± 0.6

13 ± 0.6

12 ± 0.6

11 ± 0.6

10 ± 0

W

30 ± 0

25 ± 0

22 ± 0

16 ± 0.6

13 ± 0.6

12 ± 0.6

10 ± 0.6

-

-

EA

23 ± 0.6

19 ± 1

15 ± 1

12 ± 1

11 ± 1

-

-

-

-

C

14 ± 1

10 ± 1

-

-

-

-

-

-

-

EE

27 ± 0.6

24 ± 0

22 ± 0

21 ± 0

17 ± 0

14 ± 0

11 ± 0.6

-

-

Escherichia coli ATCC 25922

M

28 ± 1

19 ± 1

15 ± 0.6

11 ± 0

10 ± 0

-

-

-

-

W

25 ± 0.6

15 ± 0.6

12 ± 0

10 ± 0

-

-

-

-

-

EA

23 ± 1

18 ± 1

15 ± 1

12 ± 0.6

-

-

-

-

-

C

12 ± 0

-

-

-

-

-

-

-

-

EE

22 ± 1

15 ± 0.6

13 ± 0.6

10 ± 0

-

-

-

-

-

  1. M, W, EA, C, and EE represents methanol, water, ethyl acetate fractions and ethanol extract, respectively, Zone of inhibition, including the diameter of the well (6 mm); mean ± SD value of three independent experiments
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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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