Fig. 3

XNJ inhibits p53 target autophagy gene expression in serum-free condition PC12 cells. PC12 cells were treated with serum or serum-free condition for 48 h in the absence or presence of XNJ. a DRAM mRNA was down-regulated by XNJ. The levels of DRAM mRNA were analyzed by QRT-PCR. b DRAM expression was down-regulated by XNJ. After XNJ treatment, the cells were lysed for Western blot analysis using an enhanced chemiluminescence system. c The density of DRAM/β-actin band among the experimental groups was compared. Data are mean ± SEM of values obtained from three independent experiments. *, p < 0.05 compared with serum-free condition