Table 1 Primer sequences used for the real-time polymerase chain reaction analysis
Gene | Primer | Amino acid sequence | Product size (bp) | Accession No. | |
|---|---|---|---|---|---|
Human | MMP1 | 5′ Primer | 5′- TAGTGGCCCAGTGGTTGAAA | 228 | NM_002421 |
3′ Primer | 5′-CCAGATTTGCCAAGAGCAGA | ||||
PKCα | 5′ Primer | 5′-CCTTTCCTTTGGAGTTTCGG | 228 | NM_002737 | |
3′ Primer | 5′-CCAACAACCTTGACCGAGTG | ||||
GAPDH | 5′ Primer | 5′- GGAGCCAAAAGGGTCATCAT | 203 | AK_026525 | |
3′ Primer | 5′- GTGATGGCATGGACTGTGGT | ||||
Mouse | MAP-2 | 5′ Primer | 5′- ACCACACCTGCAGTGGAGAA | 227 | M21041 |
3′ Primer | 5′- AATCTGGACCTGGTTCCTGC | ||||
NGF | 5′ Primer | 5′- TACTGCACCAATAGCTGCCC | 191 | NM_013609 | |
3′ Primer | 5′- TTTCAACAGGACTCACCGGA- | ||||
FSTN | 5′ Primer | 5′- GCTCTCTCTGCGATGAGCTG | 174 | NM_008046 | |
3′ Primer | 5′ ATCTCGGAAGAAACGGAGGA- | ||||
β-actin | 5′ Primer | 5′-TACAGCTTCACCACCACAGC | 187 | NM_007393 | |
3′ Primer | 5′-AAGGAAGGCTGGAAAAGAGC | ||||