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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: The Chinese medicine Sini-San inhibits HBx-induced migration and invasiveness of human hepatocellular carcinoma cells

Fig. 2

Effect of SNS on HBx-induced migration and invasion. a For the wound healing assay, HepG2-HBx-GFP cells with or without DOX were treated with different concentrations of SNS in 6-well plates and incubated for 24 h. Photomicrographs of the wounds were obtained under 100× magnification. b HepG2-HBx-GFP cells (5 × 104) were resuspended in 200 μL of DMEM, added to the upper compartment of Matrigel invasion chambers, and treated with or without DOX and different concentrations of SNS. After 24 h, the cells on the lower surface of the insert were stained with crystal violet and counted under a microscope (200× magnification). c For cell quantification, HepG2 and HepG2-HBx-GFP cells were pre-treated with 100–800 μg/ml of SNS for 24 h. Results are expressed as fold-invasion and presented as the total number of invasive cells in treated cells relative to that in untreated cells. Values represent the mean ± SEM of 3 independent experiments. *p < 0.01 compared to HBx(DOX-treated) cells

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