Table 1 Nucleotide sequences of primers used in real-time PCR
Gene (accession no.) | Primes | Nucleotiede sequences5‘-3’ | Size(bp) | Temp(°C) |
|---|---|---|---|---|
EP1 | Forward | CTGCGTCATCCATCACTT | 165 | 55.0 |
Reverse | CCAACACCACCAATACCA | 55.0 | ||
EP2 | Forward | CGGATTGTCTGGCAGTAG | 124 | 57.3 |
Reverse | GCATACAGCGAAGGTGAT | 55.0 | ||
EP3 | Forward | CTTCAATCAGATGTCAGTAGAG | 181 | 57.8 |
Reverse | CCGCTTCAGGTTGTTCAT | 55.4 | ||
EP4 | Forward | CTATACCTGCCAGACCTAAC | 174 | 56.3 |
Reverse | CTCATCCACCAACAAGACA | 57.3 | ||
GAPDH | Forward | TTCAACGGCACAGTCAAG | 116 | 55.0 |
Reverse | TACTCAGCACCAGCATCA | 55.0 |