Assessment of cytotoxicity exerted by leaf extracts from plants of the genus Rhododendron towards epidermal keratinocytes and intestine epithelial cells

Rezk, Ahmed; Al-Hashimi, Alaa; John, Warren; Schepker, Hartwig; Ullrich, Matthias S.; Brix, Klaudia

doi:10.1186/s12906-015-0860-8

BMC Complementary Medicine and Therapies

Table 2 Total cell numbers and percentages of dead cells of IEC6 and HaCaT cell cultures which were treated with three different concentrations of Rhododendron leaf extracts as indicated

From: Assessment of cytotoxicity exerted by leaf extracts from plants of the genus Rhododendron towards epidermal keratinocytes and intestine epithelial cells

Treatments	Conc. μg/mL	IEC 6		HaCaT
Treatments	Conc. μg/mL	Total cell numbers (Draq5™)	Dead cells (%)	Total cell numbers (Draq5™)	Dead cells (%)
R. hippophaeoides var. hippophaeoides	500	99 ± 46	81 ± 16	212 ± 57	0 ± 0
	50	494 ± 188	2 ± 0.6	283 ± 123	0.2 ± 0.2
	5	297 ± 139	1 ± 1	328 ± 138	2 ± 2
R. minus	500	74 ± 48	79 ± 19	221 ± 86	83 ± 12
	50	497 ± 106	0.4 ± 0.4	348 ± 113	0.4 ± 0.5
	5	683 ± 60	0.8 ± 0.2	372 ± 155	0.8 ± 0.8
R. rubiginosum	500	288 ± 120	97 ± 3	253 ± 122	4 ± 0.7
	50	551 ± 147	0.9 ± 1	413 ± 197	0.9 ± 0.9
	5	672 ± 101	0.5 ± 0.4	390 ± 41	0 ± 0
R. cinnabarinum	500	204 ± 40	100 ± 0	112 ± 23	94 ± 7
	50	475 ± 183	0.8 ± 0.8	448 ± 77	2 ± 1
	5	481 ± 128	1 ± 1	552 ± 68	2 ± 0.5
R. ferrugineum	500	33 ± 11	100 ± 0	490 ± 122	85 ± 15
	50	522 ± 62	1 ± 0.6	287 ± 109	1 ± 1
	5	439 ± 85	2 ± 2	481 ± 59	1 ± 1
R. polycladum	500	99 ± 25	100 ± 0	204 ± 62	13 ± 22
	50	274 ± 106	1 ± 1	204 ± 72	1 ± 1
	5	384 ± 104	0.9 ± 0.5	274 ± 26	0 ± 0
R. concinnum	500	304 ± 109	100 ± 0	143 ± 58	93 ± 6
	50	252 ± 58	45 ± 44	320 ± 66	0.5 ± 0.1
	5	586 ± 160	0.9 ± 0.8	260 ± 108	0 ± 0
R. xanthostephanum	500	211 ± 58	12 ± 4	127 ± 56	0 ± 0
	50	408 ± 68	0 ± 0	181 ± 70	0 ± 0
	5	460 ± 115	0.7 ± 0.6	319 ± 89	0.3 ± 0.3
R. anthopogon ssp. anthopogon	500	164 ± 41	100 ± 0	196 ± 50	90 ± 4
	50	235 ± 72	23 ± 9	370 ± 153	1 ± 1
	5	578 ± 164	2 ± 1	395 ± 157	4 ± 5
R. ambiguum	500	666 ± 220	99 ± 2	179 ± 60	0 ± 0
	50	439 ± 154	1 ± 1	327 ± 82	0.5 ± 0.6
	5	535 ± 119	0.7 ± 0.7	357 ± 150	0 ± 0
R. hirsutum	500	189 ± 65	99 ± 0.6	270 ± 113	98 ± 0.7
	50	267 ± 64	11 ± 15	258 ± 109	0.5 ± 0.1
	5	271 ± 147	1 ± 0.5	298 ± 67	0 ± 0
R. racemosum	500	224 ± 54	81 ± 23	123 ± 45	94 ± 3
	50	400 ± 262	0.4 ± 0.6	211 ± 33	0 ± 0
	5	471 ± 183	1 ± 0.7	225 ± 79	0 ± 0

Data are given as means ± standard deviation

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ISSN: 2662-7671

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