Fig. 6

Effects of EEDL on IL-6 mRNA expression and protein secretion in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were treated with the indicated concentrations of EEDL in the presence of LPS (0.2 μg/mL) for 24 h. The mRNA expression of IL-6 (a) was determined by real-time RT-PCR. Values are means ± SD (n = 3) and significant difference compared with blank control or LPS treated alone, ^##P < 0.01 vs. blank control group, *P < 0.05 vs. LPS-treated group. The protein secretion of IL-6 (b) was measured with ELISA. Values are means ± SD (n = 6) and significant difference compared with blank control or LPS treated alone, ^##P < 0.01 vs. blank control group, **P < 0.01 vs. LPS-treated group