Table 1 Primers sequences for reverse transcription–polymerase chain reaction (RT–PCR)
Gene name | Primer | Product (bp) |
|---|---|---|
(Gene bank) | (F:sense primer/R: antisense primer) | |
IL-1β | F: 5′- GTGTGTGACGTTCCCATTAGA -3′ | 101 |
(NM8361) | R: 5′- AGGTGGAGAGCTTTCAGCTCA -3′ | |
IL-6 | F: 5′- CAAGTCGGAGGCTTAAAC -3′ | 101 |
(X54542) | R: 5′- AAGTGCATCATCGTTGTTCAT -3′ | |
TNF-α | F: 5′- TCTCTACCTTGTTGCCTCCTCTTTT -3′ | 150 |
(NM013693.1) | R: 5′- TGTAGGGCAATTACAGTCACGG -3′ | |
COX-2 | F: 5′- CTGACCCCCAAGGCTCCA -3′ | 94 |
(NM011198) | R: 5′- CCAGGTCCTCGCTTATGA -3′ | |
iNOS | F: 5′- AACATCAGGTCGGCCATCA -3′ | 94 |
(NM010927) | R: 5′- CGTACCGGATGAGCTGTCA -3′ | |
β-miroglobulin | F: 5′-TTCAGTGTGAGCCAGGATATAGAAA-3′ | 153 |
(NM009735.3) | R: 5′-GAAGCCGAACATACTGAACTGCT-3′ |