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Table 2 Cytotoxicity of extracts and compounds from Nauclea pobeguinii towards sensitive and drug-resistant cancer cell lines and normal cells as determined by the resazurin assay

From: Cytotoxicity of selected Cameroonian medicinal plants and Nauclea pobeguinii towards multi-factorial drug-resistant cancer cells

Cell lines

Tested samples, IC50 values and degrees of resistance (in bracket)

Crude extracts and IC50 values (μg/mL)

IC50 values of compounds (μM)

IC50 values of doxorubicin (μM)

NPB

NPL

1

2

3

4

5

6

CCRF-CEM

14.62 ± 1.23

25.84 ± 2.16

>82.92

>243.90

>170.94

28.15 ± 1.32

25.08 ± 2.48

77.10 ± 5.87

0.20 ± 0.06

CEM/ADR5000

11.56 ± 0.85 (0.80)

25.55 ± 1.63 (0.99)

>82.92 (n.a)

>243.90 (n.a)

>170.94 (n.a)

57.43 ± 3.67 (2.04)

39.87 ± 2.91 (1.59)

>80.29 (>1.04)

195.12 ± 14.30 (975.60)

MDA-MB231

37.00 ± 2.91

64.75 ± 5.08

>82.92

>243.90

>170.94

19.90 ± 1.45

97.64 ± 7.60

>80.29

1.10 ± 0.28

MDA-MB231/BCRP

19.79 ± 2.13 (0.53)

59.55 ± 4.21 (0.92)

>82.92 (n.a)

>243.90 (n.a)

>170.94 (n.a)

22.93 ± 1.64 (1.15)

95.59 ± 8.17 (0.98)

78.26 ± 6.22 (<0.97)

7.83 ± 0.47 (7.12)

HCT116 (p53+/+)

16.19 ± 1.16

32.78 ± 2.67

>82.92

>243.90

>170.94

73.65 ± 5.67

63.77 ± 4.61

>80.29

1.41 ± 0.29

HCT116 (p53−/−)

8.70 ± 0.68 (0.54)

19.39 ± 1.34 (0.59)

>82.92 (n.a)

>243.90 (n.a)

>170.94 (n.a)

>175.36 (>2.38)

47.03 ± 4.94 (0.74)

>80.29 (n.a)

4.06 ± 0.07 (2.88)

U87MG

69.44 ± 4.34

>40

>82.92

>243.90

>170.94

76.59 ± 4.92

55.64 ± 3.73

>80.29

1.06 ± 0.15

U87MG.ΔEGFR

32.78 ± 2.79 (0.47)

63.90 ± 4.77 (>1.60)

>82.92 (n.a)

>243.90 (n.a)

>170.94 (n.a)

28.45 ± 2.06 (0.37)

47.59 ± 3.29 (0.86)

>80.29 (n.a)

6.11 ± 0.57(5.76)

AML12

>40

>40

>82.92

>243.90

>170.94

>175.36

>102.56

>80.29

>73.59

  1. (*): The degree of resistance was determined as the ratio of IC50 value in the resistant divided by the IC50 in the sensitive cell line; NPB: extract from the bark of Nauclea pobeguinii; NPL: extract from the leaves of Nauclea pobeguinii; Compounds isolated from NPB [1: 3-acetoxy-11-oxo-urs-12-ene; 2: p-coumaric acid; 3: citric acid trimethyl ester; 4: resveratrol; 5: resveratrol β-D-glucopyranoside; 6: strictosamide]; n.a.: not applicable. IC50 value are mean ± SD of at least two experiments with six replicates each