Fig. 4
Effect of Calycosin on LPS-induced dysfunction of cell migration. a HE staining of migrated HUVECs. Cells were treated with 0.2 μg/ml LPS in the presence of 10 μg/ml Calycosin, or 50 umol/l Y27632, or10−5 mol/l valsartan for 0.5 h. After that, the migrated cells on the bottom side were stained with HE staining for counting migrated cells with a 10X objective lens, and cell morphology was photographed. b The amount of migrated cells. △P < 0.05, compared with normal HUVECs; ★P < 0.05, compared with LPS-induced inflammatory and injured HUVECs