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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Calycosin-7-O-β-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells

Fig. 2

The content of cNOS, tNOS, iNOS and NO in cell supernatants. The content of cNOS, tNOS, iNOS and NO in cell supernatants was determined by nitrate reductase method. a The content of cNOS in the supernatant was assayed after HUVECs were incubated with calycosin in different concentration (0 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml or 20 μg/ml) for 24 h; b The content of cNOS in the supernatant was assayed after HUVECs were incubated with 10 μg/ml calycosin for different time (0 h, 4 h, 8 h, 12 h, 24 h or 48 h); c The content of cNOS, tNOS and iNOS in cell supernatants; d The content of NO in cell supernatants. P < 0.05, compared with normal HUVECs; P < 0.05, compared with LPS-induced inflammatory and injured HUVECs

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