Fig. 3

Quantitative real time PCR: Quantitative real timePCR evaluation on expression of tumor iNOS, NF-kB, c-MYC and iCAM of MK (50 and 200 mg/kg) treated and untreated mice. The statistical differences among all groups were assessed by one-way ANOVA followed by Duncan post-hoc. The differences between the control or treated group and untreated group were determined by one-way ANOVA (∗p < 0.05). Normal: normal healthy mice; LR untreated: untreated low risk mice inoculated with 1 × 10⁴4T1 cell/mice; LR MK 50 mg/kg: low risk mice treated with 50 mg/kg of MK extract for 30 days before inoculated with 1 × 10⁴4T1 cell/mice; LR MK 200 mg/kg: low risk mice treated with 200 mg/kg of MK extract for 30 days before inoculated with 1 × 10⁴4T1 cell/mice; HR untreated: untreated high risk mice inoculated with 1 × 10⁶4T1 cell/mice; HR MK 50 mg/kg: high risk mice treated with 50 mg/kg of MK extract for 30 days before inoculated with 1 × 10⁶4T1 cell/mice; HR MK 200 mg/kg: high risk mice treated with 200 mg/kg of MK extract for 30 days before inoculated with 1 × 10⁶4T1 cell/mice inoculated mice. LR: Low risk groups. HR: High risk groups