Antioxidant potential, total phenolic and total flavonoid contents of Rhododendron anthopogonoides and its protective effect on hypoxia-induced injury in PC12 cells

Table 2 Effect of crude extract and solvent fractions of R. anthopogonoides on different free radical scavenging assays

Extract/fractions	EC₅₀ value (μg/ml) of radical scavenging
Extract/fractions	DPPH racidal	ABTS radical	Superoxide radical	Hydroxyl radical	Nitric oxide radical
Crude extract	63.73 ± 0.95^d	69.96 ± 0.92^c	483.00 ± 2.65^d	204.65 ± 4.26^c	197.38 ± 4.25^d
Hexane	259.65 ± 13.04^e	168.64 ± 2.50^d	>1000	468.93 ± 16.17^f	>1000
Ethyl acetate	61.78 ± 0.11^c	53.36 ± 0.53^b	433.33 ± 7.68^b	240.39 ± 2.50^d	183.83 ± 2.34^c
n-butanol	53.11 ± 0.12^b	71.32 ± 0.50^c	270.67 ± 0.58^a	145.87 ± 0.83^b	151.34 ± 3.67^b
water	278.35 ± 1.4^e	345.22 ± 12.62^e	>1000	232.95 ± 2.87^e	>1000
Ascorbic acid	30.39 ± 0.23^a	18.51 ± 0.13^a	450.32 ± 6.28^c	50.73 ± 1.83^a	126.72 ± 7.24^a

Each value represents as mean ± SD of triplicate experiments. Values in the same column followed by a different letter (a–f) are significantly different (p < 0.05)

ISSN: 2662-7671