Fig. 3

Effect of oral administration of the methanol/methylene chloride extract of the stem bark of Croton macrostachyus (MECM) on the mechanical hyperalgesia evaluated with von Frey hair (0.4 g) in PGE₂ (0.1 nmol/paw) inflamed mice paw. In panel (a), animals were treated 1 hour before PGE₂ injection and pain response was evaluated before treatment and after PGE₂ injection. In panel (b) and (c), treatments were given orally 1 hour after PGE₂ and response to pain was evaluated before PGE₂ injection and after treatment. N = 5; ^ap < 0.05; ^bp < 0.01; ^cp < 0.001 significantly different compared to vehicle. B = basal response to mechanical stimulation