| Â | HBV DNA by Southern blottinga | HBV DNA by qPCRb | HBs Ag | HBc Ag |
|---|
| Â | EC50c | EC90d | SIe | EC50c | EC90d | SIe | EC50c | SIe | EC50c | SIe |
HBV WT | 78.6 ± 1.3 | 154.8 ± 11.8 | 9.63 | 119.8 ± 21.2 | 228.2 ± 11.8 | 6.31 | 252.5 ± 27.0 | 2.99 | 185.9 ± 30.1 | 4.09 |
YIDD | 82.3 ± 18.6 | 183.1 ± 22.3 | 9.19 | 125.2 ± 18.6 | 234.7 ± 22.3 | 6.04 | 239.5 ± 16.3 | 3.16 | 234.4 ± 19.3 | 3.23 |
YVDD | 104.6 ± 9.2 | 179.4 ± 32.4 | 7.23 | 126.1 ± 9.2 | 228.2 ± 11.8 | 6.00 | 263.7 ± 10.4 | 2.87 | 241.3 ± 25.3 | 3.14 |
YSDD | 90.1 ± 13.2 | 192.3 ± 22.6 | 6.91 | 139.8 ± 33.7 | 242.6 ± 32.4 | 5.4 | ND | ND | 195.6 ± 20.2 | 3.87 |
- Each value represents the mean ± SD from three independent experiments. HBV DNA was extracted from isolated intracellular core particles after various concentrations of Phyllanthus urinaria koreanis extract treatment for 48 h from HBV WT and mutant-transfected HepG2 cells
- ND not determined
- aHBV DNA level was measured by Southern blotting using Fujifilm Image Gauge V 4.0 program
- bHBV DNA level was measured by real-time quantitative PCR. Primers for pRCR are listed in Additional file 1
- cEC50 is the concentration at which 50Â % depression of HBV DNA and HBs/e Ag secretion was observed
- dEC90 is the concentration at which 90Â % depression of HBV DNA was observed
- eSelectivity index (SI) was calculated by EC50/CC50. CC50 of extract on HepG2 cells was 757.0 ± 56.5 μg/ml.CC50, the drug concentration that reduced cell viability to 50 % of that the control was determined by MTT assay
