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Table 1 Phyllanthus urinaria koreanis extract inhibits replication of HBV WT and LMV-resistant mutants

From: Inhibitory effect of Phyllanthus urinaria L. extract on the replication of lamivudine-resistant hepatitis B virus in vitro

  HBV DNA by Southern blottinga HBV DNA by qPCRb HBs Ag HBc Ag
  EC50c EC90d SIe EC50c EC90d SIe EC50c SIe EC50c SIe
HBV WT 78.6 ± 1.3 154.8 ± 11.8 9.63 119.8 ± 21.2 228.2 ± 11.8 6.31 252.5 ± 27.0 2.99 185.9 ± 30.1 4.09
YIDD 82.3 ± 18.6 183.1 ± 22.3 9.19 125.2 ± 18.6 234.7 ± 22.3 6.04 239.5 ± 16.3 3.16 234.4 ± 19.3 3.23
YVDD 104.6 ± 9.2 179.4 ± 32.4 7.23 126.1 ± 9.2 228.2 ± 11.8 6.00 263.7 ± 10.4 2.87 241.3 ± 25.3 3.14
YSDD 90.1 ± 13.2 192.3 ± 22.6 6.91 139.8 ± 33.7 242.6 ± 32.4 5.4 ND ND 195.6 ± 20.2 3.87
  1. Each value represents the mean ± SD from three independent experiments. HBV DNA was extracted from isolated intracellular core particles after various concentrations of Phyllanthus urinaria koreanis extract treatment for 48 h from HBV WT and mutant-transfected HepG2 cells
  2. ND not determined
  3. aHBV DNA level was measured by Southern blotting using Fujifilm Image Gauge V 4.0 program
  4. bHBV DNA level was measured by real-time quantitative PCR. Primers for pRCR are listed in Additional file 1
  5. cEC50 is the concentration at which 50 % depression of HBV DNA and HBs/e Ag secretion was observed
  6. dEC90 is the concentration at which 90 % depression of HBV DNA was observed
  7. eSelectivity index (SI) was calculated by EC50/CC50. CC50 of extract on HepG2 cells was 757.0 ± 56.5 μg/ml.CC50, the drug concentration that reduced cell viability to 50 % of that the control was determined by MTT assay