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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Effect of Qing’e formula on the in vitro differentiation of bone marrow-derived mesenchymal stem cells from proximal femurs of postmenopausal osteoporotic mice

Fig. 2

Proliferation and morphology of cultured BMSCs after treatment with QEF-containing serum. The level of cellular proliferation, determined by counting the number of cells, was visually monitored in cells cultured in serum treated with water (control, group A); a low concentration of QEF (group B); a medium concentration of QEF (group C); and a high concentration of QEF (group D). During this time, there was no significant difference observed between groups A and B (P > 0.05), while groups C and D were significantly higher than A and B from day 5 onward (P < 0.05) (a). Values are expressed as the mean ± SD; P < 0.05 compared to group A at the specified time point, P < 0.05 compared to group B at the specified time point. All groups reached a growth plateau between culture day 10 and 11. Representative images of the cellular morphology at day 10 are shown for group A (b), group B (c), group C (d), and group D (e). All photomicrograph images were obtained at a ×100 magnification

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