Fig. 1

Schematic drawing of in vivo experimental procedures. In study 1 and study 2, the mice (5 wks old) were randomly assigned to the various experimental treatments that were administered daily for 10 wks. An IP-GTT was performed at 0, 5 and 10 wks of the study, while an IP-ITT was performed at 10 wks. In each intervention blood was collected at 0 and 15 min post injection to detect insulin plasma levels