Fig. 2

Portulaca oleracea ethanol extract (POEE) reduces receptor-mediated Ca²⁺ release from internal Ca²⁺ stores, but not extracellular Ca²⁺ influx. Isolated bone marrow-derived macrophages (BMMs) were plated on cover glass and cultured for 48 h in the presence of receptor activator of NF-κB ligand (RANKL) (50 ng/mL). After incubation, free cytosolic Ca²⁺ ([Ca²⁺]_i) was measured. a To deplete internal Ca²⁺ stores, extracellular Ca²⁺ was removed by adding 1 mM of EGTA, and BMMs were then treated with 25 μM of cyclopiazonic acid. Following Ca²⁺ store depletion, 1 mM of Ca²⁺ was added back in the presence or absence of POEE. b Cells were treated with a low concentration of adenosine triphosphate (ATP) (10 μM) in the absence of extracellular Ca²⁺. Extracellular Ca²⁺ was then restored to determine receptor-mediated extracellular Ca²⁺ influx. ΔCa²⁺ increase (indicated with an arrow) is presented as relative mean fold change