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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Attenuated RANKL-induced cytotoxicity by Portulaca oleracea ethanol extract enhances RANKL-mediated osteoclastogenesis

Fig. 2

Portulaca oleracea ethanol extract (POEE) reduces receptor-mediated Ca2+ release from internal Ca2+ stores, but not extracellular Ca2+ influx. Isolated bone marrow-derived macrophages (BMMs) were plated on cover glass and cultured for 48 h in the presence of receptor activator of NF-κB ligand (RANKL) (50 ng/mL). After incubation, free cytosolic Ca2+ ([Ca2+]i) was measured. a To deplete internal Ca2+ stores, extracellular Ca2+ was removed by adding 1 mM of EGTA, and BMMs were then treated with 25 μM of cyclopiazonic acid. Following Ca2+ store depletion, 1 mM of Ca2+ was added back in the presence or absence of POEE. b Cells were treated with a low concentration of adenosine triphosphate (ATP) (10 μM) in the absence of extracellular Ca2+. Extracellular Ca2+ was then restored to determine receptor-mediated extracellular Ca2+ influx. ΔCa2+ increase (indicated with an arrow) is presented as relative mean fold change

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