Figure 2

The HPLC chromatograms of (a) mixture standard solutions including 4 mg/ml fructose, 3 mg/ml glucose and 1 mg/ml sucrose, and (b) honey samples (9.4 mg/ml). Conditions: High-performance liquid chromatographic analysis was carried out using a Shimadzu 20A series HPLC system (Japan) equipped with two pumps with column oven and ultraviolet detector; a Welch ultimate XB-NH₂ column (4.6 mm × 250 mm, 5 μm) was used, sample injection volume was 20 μl, the temperature of column oven was set at 30 °C, flow rate was 1 ml/min, a isocratic elution was performed with mobile phases consisted of water and acetonitrile (30 : 70) and the monitored wavelength was set at 190 nm