Fig. 8
H2O2 concentration in culture medium generated from D-glucose after addition of 5, 8, 10 and 25-mU/ml glucose oxidase (GOx) during 48 h. GOx was added to the medium of culture wells (a) without or (b) with confluent cultures of ARPE-19 cells. Because higher medium concentrations are achieved in the absence of cells, different y-axis scales are used. Data are from three independent experiments and are the means ± SD of three culture wells per group in each experiment