Fig. 12

The effect of hydrogen peroxide by enzymatic generation (GOx 50, 25 and 12.5 mU/ml) and pulse (1600, 1000, 800 μM) in presence or absence of co-treatment with Bucida buceras ethanol extract (1600 μg/ml) on catalase activity (a) and GSH-peroxidase activity assay (b) H₂O₂ (initially, 200 μM in PBS was incubated for 1 h, at 37 °C with different cell lysates, control GOx (50 mU/ml + catalase 200 U/ml) or control cells (untreated with H₂O₂). Results are expressed as the means ± SD of three independent studies (each analyzed in triplicate). *p < 0.05 significantly different from value of cells control (untreated) vs. H₂O₂-treated **p < 0.05 significantly different from value of antioxidant treatment vs. H₂O₂-treated, n.s not significant differences