Fig. 11
Effects of GOx and H2O2 in addition on glutathione reduced (GSH) at 24 h (a). ARPE-19 cells treated with 12.5, 25 and 50 mU/ml of GOx and pulse of H2O2 800, 1000, 1600 μM and ARPE-19 cells treated with 1600 ug/ml of ethanolic extract of Bucida buceras, control cells (untreated with H2O2) or GOx (50 mU/ml) + 200 U/ml of catalase as CAT control; (b) The levels of H2O2 in GOx at 24 h in 12.5, 25 and 50 mU/ml, the medium was analyzed using FOX reagent. Values were expressed in means ± SD in μM (n = 3 independent experiments). *p < 0.05 significantly different from value of cells control vs. H2O2-treated **p < 0.05 significantly different from value of antioxidant treatment vs. H2O2-treated, n.s not significant differences