Table 4 Factorial analysis [2 × 2 factorial design (untreated/treated, treatment for 7/28 d)] of IL-6, IL-17, IL-23, TGF-β levels and RORγt, Foxp3 expression between the MDL and PCF groups
From: Pingchuan formula improves asthma via restoration of the Th17/Treg balance in a mouse model
Variation | Indicator | SS | df | MS | F | P |
|---|---|---|---|---|---|---|
Group (difference between MDL and PCF group) | IL-6 | 38352.38 | 1 | 38352.38 | 131.94 | <0.001 |
IL-17 | 7712.34 | 1 | 7712.34 | 89.54 | <0.001 | |
IL-23 | 128013.36 | 1 | 128013.36 | 227.20 | <0.001 | |
TGF-β | 99794.91 | 1 | 99794.91 | 38.07 | <0.001 | |
RORγt | 0.21 | 1 | 0.21 | 16.11 | <0.001 | |
Foxp3 | 0.42 | 1 | 0.42 | 57.65 | <0.001 | |
Time (difference between 7 d and 28 d treatment) | IL-6 | 377.41 | 1 | 377.41 | 1.30 | 0.262 |
IL-17 | 261.65 | 1 | 261.65 | 3.04 | 0.090 | |
IL-23 | 2497.38 | 1 | 2497.38 | 4.43 | 0.042 | |
TGF-β | 26216.14 | 1 | 26216.14 | 10.00 | 0.003 | |
RORγt | 0.02 | 1 | 0.02 | 1.42 | 0.241 | |
Foxp3 | 0.00 | 1 | 0.00 | 0.08 | 0.786 | |
Group *Time | IL-6 | 1198.32 | 1 | 1198.32 | 4.12 | 0.050 |
IL-17 | 308.50 | 1 | 308.50 | 3.58 | 0.066 | |
IL-23 | 7075.54 | 1 | 7075.54 | 12.56 | 0.001 | |
TGF-β | 20936.21 | 1 | 20936.21 | 7.99 | 0.008 | |
RORγt | 0.00 | 1 | 0.00 | 0.08 | 0.783 | |
Foxp3 | 0.02 | 1 | 0.02 | 2.85 | 0.100 | |
SE | IL-6 | 10464.63 | 36 | 290.68 | -- | -- |
IL-17 | 3100.86 | 36 | 86.16 | -- | -- | |
IL-23 | 20284.27 | 36 | 563.45 | -- | -- | |
TGF-β | 94369.00 | 36 | 2621.36 | -- | -- | |
RORγt | 0.46 | 36 | 0.01 | -- | -- | |
Foxp3 | 0.26 | 36 | 0.01 | -- | -- |