Table 3 The oligonucleotides used as primers
Gene | Primer name | Sequence of primer | Target length |
|---|---|---|---|
tnfα | rTNFaF | CTTCTCATTCCTGCTCGTGG | 140 bp |
rTNFaR | ATCTGAGTGTGAGGGTCTGGG | ||
IL-6 | rIL6F | AAGCCAGAGTCATTCAGAGCAA | 160 bp |
rIL6R | TGGATGGTCTTGGTCCTTAGC | ||
IL-10 | rIL10F | CCTGGTAGAAGTGATGCCCC | 163 bp |
rIL10R | ATTCTTCACCTGCTCCACTGC | ||
AIF | rAIFF | CTGAGAAAGGAAATATGGGGAA | 138 bp |
rAIFR | TGAGTAACTTGCCACCGCTG | ||
P53 | rP53F | GGAAGGAAATCCGTATGCTGA | 177 bp |
rP53R | GTGATGATGGTAAGGATGGGC | ||
Bax | rBaxF | CCCGAGAGGTCTTCTTCCG | 167 bp |
rBaxR | GAAGTCCAGTGTCCAGCCCA | ||
caspase-3 | rcaspase-3 F | CGAAACTCTTCATCATTCAGGC | 129 bp |
rcaspase-3R | AGTAAGCATACAGGAAGTCGGC | ||
Bdnf | rBdnfF | CAGCGGCAGATAAAAAGACTG | 187 bp |
rBdnfR | GTAGTTCGGCATTGCGAGTTC | ||
TrkB | rTrkBF | TATGAAGACTGGACCACGCC | 167 bp |
rTrkBR | AGAAGCAGCATCACCAGCAG | ||
CREB | rCREBF | AGACCACTGATGGACAGCAGAT | 150 bp |
rCREBR | TAGGAAGTGCTGGGGAGGAC | ||
Bcl-2 | rBcl-2 F | GTGAACTGGGGGAGGATTGT | 167 bp |
rBcl-2R | GCATCCCAGCCTCCGTTA | ||
actin | rat actin f | CCCATCTATGAGGGTTACGC | 150 bp |
rat actin r | TTTAATGTCACGCACGATTTC |