Fig. 3

Protective effect of PMC-12 on types of cell death in HT22 cells. Cells were pretreated with 0.1, 1, or 10 μg/ml PMC-12 for 24 h, followed by exposure to 5 mM glutamate for 24 h. Representative flow cytometric analysis scatter-grams of Annexin V/PI staining (a) and quantitative analysis of the histograms (b and c). ^###P < 0.001 vs. control; ***P < 0.001 vs. glutamate-treated cells. All data are represented as the mean ± SEM of three independent experiments