Fig. 4
Haelan-induced apoptosis in pancreatic carcinoma cells and fibroblasts. CAPAN-1 cells a, BxPC-3 cells b, AR42J cells c and fibroblasts d were incubated with 8 % Haelan or vehicle in the presence or absence of the calpain inhibitor PD150606 or Ca2+ chelator BAPTA-AM and stained after 24 h using the CytoGlo Annexin V-FITC Apoptosis Detection kit. The percentages of Annexin V-positive cells are assessed by flow cytometric analyses. Haelan induces apoptosis in all cell types investigated. PD150606 or BAPTA-AM increases the degree of Haelan-induced apoptosis in all cells with the exception of the fibroblasts. The results are expressed as mean + SEM (n = 3-4). *P < 0.05, **P < 0.01, control vs. Haelan-treated cells, xP < 0.05, Haelan-treated cells vs. cells treated with Haelan in the presence of PD150606 or BAPTA-AM