Fig. 3

The suppression of LPS-induced signaling by pretreatment of XYXD in HSC. a, NF-κB luciferase was measured. One hour after pretreatment with XYXD (5ug/ml and 25 ug/ml), GFP-Col-HSC cells were treated with LPS (100 ng) for 4 h and then transfected NF-κB-luciferase reporter plasmid for 16 h. *P < 0.05, * *P < 0.01, vs Un-LPS and No XYXD, ^#P < 0.05, ^##P < 0.01, vs LPS and No XYXD. b, Western blot analysis for p-JNK, total JNK, p-p38 and total p38. One hour after pretreatment with XYXD (5 μg/ml and 25 μg/ml), GFP-Col-HSC cells were treated with LPS (100 ng) for 30 min. c, the mRNA expression of TIMP1 and Bambi was detected by real-time PCR. *P < 0.05, * *P < 0.01, vs vehicle, ^#P < 0.05, ^##P < 0.01, vs LPS. d, the mRNA expression of CCL2 and CXCL2 was detected by real-time PCR. One hour after pretreatment with XYXD (5 μg/ml and 25 μg/ml), GFP-Col-HSC cells were treated with LPS (100 ng) for 4 h. *P < 0.05, * *P < 0.01, vs vehicle, ^#P < 0.05, ^##P < 0.01, vs LPS. Data represent the mean ± SD of triplicate cultures. A representative result is shown. Similar results were obtained in three independent experiments