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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Xia-yu-xue decoction (XYXD) reduces carbon tetrachloride (CCl4)-induced liver fibrosis through inhibition hepatic stellate cell activation by targeting NF-κB and TGF-β1 signaling pathways

Fig. 3

The suppression of LPS-induced signaling by pretreatment of XYXD in HSC. a, NF-κB luciferase was measured. One hour after pretreatment with XYXD (5ug/ml and 25 ug/ml), GFP-Col-HSC cells were treated with LPS (100 ng) for 4 h and then transfected NF-κB-luciferase reporter plasmid for 16 h. *P < 0.05, * *P < 0.01, vs Un-LPS and No XYXD, #P < 0.05, ##P < 0.01, vs LPS and No XYXD. b, Western blot analysis for p-JNK, total JNK, p-p38 and total p38. One hour after pretreatment with XYXD (5 μg/ml and 25 μg/ml), GFP-Col-HSC cells were treated with LPS (100 ng) for 30 min. c, the mRNA expression of TIMP1 and Bambi was detected by real-time PCR. *P < 0.05, * *P < 0.01, vs vehicle, #P < 0.05, ##P < 0.01, vs LPS. d, the mRNA expression of CCL2 and CXCL2 was detected by real-time PCR. One hour after pretreatment with XYXD (5 μg/ml and 25 μg/ml), GFP-Col-HSC cells were treated with LPS (100 ng) for 4 h. *P < 0.05, * *P < 0.01, vs vehicle, #P < 0.05, ##P < 0.01, vs LPS. Data represent the mean ± SD of triplicate cultures. A representative result is shown. Similar results were obtained in three independent experiments

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