Stage | Stage description | PTZ Concentration at which the behavior is first seen |
---|---|---|
1 | Stay or move near the bottom of the tank | 0.10 mg/ml (0.72 mM) |
2 | Rapid movement in all directions, | 0.10 mg/ml (0.72 mM) |
3 | Hit the tank walls | 0.50 mg/ml (3.6 mM) |
4 | Rapidly go to the water surface and then rapidly go to the bottom of the tank | 0.70 mg/ml (5.0 mM) |
5 | Shaking movement | a |
6 | Erratic movements (circular motion, diagonal movement) | 0.70 mg/ml (5.0 mM) |
7 | Wild jumping, Clonic-like movement (convulsion) then loss of coordination/ posture, remaining at the bottom | 0.70 mg/ml (5.0 mM) |
8 | Recover posture/motion | 0.70 mg/ml (5.0 mM) |
9 | Death | 1 mg/mlb (7.5 mM) |