Fig. 5

Immunohistochemical staining of cyclin D1. The expression of cyclin D1 in three sections, including distal, middle, and proximal colon in normal (Group 1), AOM alone (untreated) (Group 2) compared to groups of rats treated with 10 % (Group 3), 20 % (Group 4), and 40 % (Group 5) (w/w) of brewers’ rice (n = 3). Expression of cyclin D1 showed cytoplasm and nucleus staining: strong cyclin D1 in Group 2, weaker staining of cyclin D1 in Group 3 and Group 4, and the weakest staining of cyclin D1 in Group 5 (Magnification 200×). Scale bars in the figures indicate 100 μm