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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: Oligonol suppresses lipid accumulation and improves insulin resistance in a palmitate-induced in HepG2 hepatocytes as a cellular steatosis model

Fig. 1

Oligonol attenuated PA-induced increase in intracellular lipid content in HepG2 cells. (a) Oligonol cytotoxicity measurement. HepG2 cells were incubated with various concentrations of Oligonol (OLG; ranging from 0 to 50 μg/ml) for 24 or 48 h, and cell viability was measured by MTT assay. (b and c) Cells were treated with 250 μM of palmitate (PA) in the absence or presence of Oligonol (ranging from 1 to 10 μg/ml) for 24 h, and stained with Oil-red O to visualize the intracellular lipid contents. (b) Lipid contents were quantified by its OD value at 500 nm. Data were presented as mean ± SEM of three independent experiments; each performed in triplicates. ###P < 0.005 vs. vehicle-treated control; ** P < 0.01, and *** P < 0.005 vs. palmitate-treated cells (PA). (c) Micrographs of phase contrast microscopy. Control (CTL) is vehicle treated cells. Scale bar is 100 μm

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